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Merck

HPLC Analysis of Hemoglobin S on Discovery® BIO PolyMA-SCX, Ion Exchange

HPLC Analysis of Hemoglobin S on Discovery® BIO PolyMA-SCX, Ion Exchange application for HPLC

Materiales

Standard

Referencia del producto
Descripción
Precios

Hemoglobin human

lyophilized powder

Hemoglobin S, Ferrous Stabilized human

lyophilized powder

CONDITIONS

column

Discovery BIO PolyMA-SCX, 5cm × 4.6mm, 5μm particles (59601-U)

mobile phase

[A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0

gradient

0 to 100% B in 30 min. (linear)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

10 μL

Descripción

Analysis Note

Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany