Saltar al contenido
Merck

In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.

Methods in molecular biology (Clifton, N.J.) (2022-02-12)
Pau Marfull-Oromí, Kathryn M Miller, Yimin Zou
RESUMEN

In vitro studies have provided valuable insights to the function and mechanisms in axon guidance. In this chapter, we will introduce the rodent "open-book" assay, pre- or postcrossing explant culture and the dissociated neuron culture. They have been used to discover mechanism which we have gone on to validate or will confirm using in vivo genetic approaches.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Forskolina, For use in molecular biology applications
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Roche
Collagen, from rat tail tendon
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
SB 216763, >98% (HPLC)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Cloruro de litio, BioXtra, ≥99.0% (titration)
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
Gö 6976, Gö 6976, CAS 136194-77-9, is a cell-permeable, reversible, and ATP-competitive inhibitor of PKC (IC₅₀ = 7.9 nM for rat brain). Exhibits selectively for PKCα (IC₅₀ = 2.3 nM) and βI (IC₅₀ = 6.2 nM).
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Sigma-Aldrich
JNK Inhibitor I, (L)-Form, Cell-Permeable, The JNK Inhibitor I, (L)-Form, Cell-Permeable controls the biological activity of JNK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
En este momento no podemos mostrarle ni los precios ni la disponibilidad