G1007613

LC/MS/MS Analysis of Pesticides in Cannabis on the Ascentis^® RP-Amide after QuEChERS Extraction and Cleanup Using Supel^™ QuE Verde

application for LC-MS, application for SPE

Propiedades

• technique(s): LC/MS: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: Dispersive (QuEChERS); sample/matrix: 1.9 g of cannabis spiked with pesticides at 50 ng/g; extraction tube: Supel QuE Citrate extraction tube (55227-U); extraction process: add 10 mL of acetonitrile; shake for 30 minutes at 2500 rpm on shaker; add contents of Supel QuE citrate extraction tube; (shake immediately for 1 minute; centrifuge at 5000 rpm for 5 minutes); clean-up tube: Supel QuE Verde, 2 mL (55227-U); clean-up process: transfer 1 mL of acetonitrile layer into Supel QuE Verde cleanup tube; shake for 1 minutes; (centrifuge at 5000 rpm for 3 minutes; draw of supernatant for LC/MS/MS analysis); column: Ascentis RP-Amide, 10 cm x 2.1 mm I.D., 3.0 μm particles (565301-U); column: Ascentis RP-Amide Supelguard guard cartridge, 2 cm x 2.1 mm I.D., 5 μm particles (565372-U); mobile phase: [A] 5 mM ammonium formate, 0.1% formic acid in 95:5 water:acetonitrile; [B] 5 mM ammonium formate, 0.1% formic acid in 5:95 water:acetonitrile; gradient: 10% B held for 1 min; to 100% B in 13 min; held at 100% B for 6 min; to 10% in 0.5 min; held at 10% B for 6 min; flow rate: 0.4 mL/min; column temp.: 30 °C; detector: MRM; injection: 5 μL; sample: QuEChERS extract of cannabis in acetonitrile
• suitability: application for LC-MS; application for SPE
• application(s): environmental

Descripción

Analysis Note

Consumption of cannabis and/or cannabis based products is currently legal in some form in 28 US states plus the District of Columbia. Pesticide testing on plant material and extracts is required in some states, including Oregon, which mandates a specific target list for testing. A common approach used by testing labs for pesticides is QuEChERS extraction followed by a dispersive cleanup. In this application a majority of the LC-amenable pesticides on the Oregon list were extracted from cannabis spiked at 50 ng/g using QuEChERS, followed by cleanup with Supel^™ QuE Verde. Analysis was achieved using the Ascentis^® RP-Amide column equipped with a guard. Supel QuE Verde offered sufficient background reduction for LC/MS/MS analysis with recoveries of 70-120% for a majority of the pesticides.

Other Notes

Sample pretreatment: Added 10 mL of deionized water, mixed and equilibrated for 10 minutes

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC

Analito

Daminozide
Acephate
Oxamyl
Methomyl
Thiamethoxam
Flonicamid
Imidacloprid
Dimethoate
Acetamiprid
Aldicarb
Thiacloprid
Imazalil (Enilconazole)
Dichlorvos
Propoxur
Spiroxamine
Carbofuran
Metalaxyl
Carbaryl
Spinosyn A
Spinosyn D
Spirotetramat
Paclobutrazol
Chlorantraniliprole
Methiocarb (Mercaptodimethur)
Myclobutanil
Azoxystrobin
Ethoprop (Ethoprophos)
Phosmet (Imidan)
Tebuconazole
Boscalid (Nicobifen)
Bifenazate (D 2341)
Malathion
Propiconazole
Fenoxycarb
Kresoxim methyl
Clofentezin
Prallethrin
Trifloxystrobin
Piperonyl butoxide
Fenpyroximate (E)
Hexylthiazox
Etoxazole
Spiromesifen
Pyrethrin
Pyridaben
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