G005880

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Grilled Hamburger on SPB^®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel^™ QuE Z-Sep, Fast GC Analysis

suitable for GC, application for SPE

Propiedades

• technique(s): gas chromatography (GC): suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: Dispersive (QuEChERS); sample/matrix: grilled hamburger spiked with PAHs at 100 ng/g; extraction tube: Sodium chloride/magnesium sulfate (55294-U); extraction process: add 25 mL acetonitrile, contents of unbuffered salt tube (55294-U); shake for 5 minutes; centrifuge for 5 minutes at 3200 rpm; clean-up tube: Supel QuE Z-Sep tube (55403-U); clean-up process: transfer 3 mL of acetonitrile layer into a Supel QuE Z-Sep cleanup tube (55403-U); shake 1 minute; centrifuge for 3 minutes at 3400 rpm; remove 1 mL supernatant for GC-MS analysis; column: SPB-608, 20 m x 0.18 mm I.D. x 0.18 μm; oven: 60 °C (1 min), 25 °C/min to 275 °C, 10 °C/min to 300 °C (13 min); inj. temp.: 265 °C; detector: MS-SIM; MSD interface: 300 °C; carrier gas: helium, 1.5 mL/min constant flow; injection: 1 μL splitless (1 min.); liner: 4 mm I.D. FocusLiner^™ with taper; sample: grilled hamburger extract
• suitability: application for SPE
• application(s): food and beverages

Descripción

Analysis Note

Sample pretreatment: Place 5 g ground hamburger sample into 50 mL centrifuge tube (55248-U). Spike with 100 ng/g target PAHs and internal standards. Let sit 10 min. Add 5 mL water and homogenize.

This application demonstrates the use of dispersive SPE or QuEChERS for the extraction and cleanup of grilled hamburger for GC-MS analysis of polynuclear aromatic hydrocarbons (PAHs). Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >80% for a majority of the PAHs tested.

Other Notes

Study Schedule

Legal Information

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.

SPB is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC