567513-U

Discovery^® HS F5 (5 µm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

• Sinónimos: Discovery Pentafluorophenyl PFP HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Propiedades

• product name: Columna para HPLC Discovery^® HS F5, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• agency: suitable for USP L43
• product line: Discovery^®
• feature: endcapped
• manufacturer/tradename: Discovery^®
• packaging: 1 ea of
• extent of labeling: 12% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 5 cm × 4.6 mm
• surface area: 300 m²/g
• surface coverage: 4 μmol/m²
• impurities: <10 ppm metals
• matrix: silica gel, high purity, spherical particle platform; fully porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 5 μm
• pore size: 120 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Descripción

General description

Características de la Discovery^® HS F5:
• Selectividad única
• Retención similar a la C18 (algunas veces requiere una fase móvil más fuerte)
• Excelente forma del pico
• Estable, escaso sangrado en separaciones de CL/EM (LC/MS)
• Separaciones modulables de 3 a 10μm

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Application

• Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study utilizes the Discovery^® HS F5 HPLC Column to develop an HPLC-UV method for the simultaneous determination of phenylalanine and tyrosine in blood, significantly advancing the diagnostic and monitoring capabilities for phenylketonuria (Rabti et al., 2024).


• A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities.: Demonstrates the application of the Discovery^® HS F5 HPLC Column in optimizing sample preparation techniques for the detection of pharmaceuticals and personal care products in environmental samples, enhancing the analysis of diverse hydrophilic compounds (Portillo-Castillo et al., 2021).


• "Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.: Utilizes the Discovery^® HS F5 HPLC Column in a novel bidimensional chromatographic setup to study the stereoselective metabolism of CNS-targeting chiral drugs, contributing to a deeper understanding of their pharmacological profiles (Battisti et al., 2016).


• HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).: This research employs the Discovery^® HS F5 HPLC Column for developing an HPLC method to identify and characterize impurities in the antitumor drug LMP776, ensuring drug purity and compliance with regulatory standards (Wang et al., 2016).


• Development studies on determination of preservatives decomposition products.: Showcases the application of the Discovery^® HS F5 HPLC Column in the analysis of decomposition products of preservatives, enhancing the safety assessments of food and pharmaceutical products (Zabrzewska et al., 2014).

Features and Benefits

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany