ZRB1761

Anti-ZO-1 Antibody, clone 4E14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 4E14, monoclonal; recombinant monoclonal
• Línea del producto: ZooMAb^® learn more
• Formulario: lyophilized
• mol peso: calculated mol wt ~195.46 kDa; observed mol wt ~250 kDa
• reactividad de especies: human
• reactividad de especies (predicha por homología): monkey
• envase: antibody small pack of 25 μL
• características de los productos alternativos más sostenibles: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validación mejorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnicas: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso UniProt: Q07157
• categoría alternativa más sostenible: , Aligned
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Especificidad

Clone 4E14 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Tight junction protein ZO-1.

Inmunógeno

GST/His-tagged recombinant fragment corresponding to 145 amino acids from the N-terminal half of human Tight junction protein ZO-1 (ZO1).

Aplicación

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ZO-1 in A549 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in HepG2, MCF-7, and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ZO-1 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZO-1 in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ZO-1, clone 4E14 ZooMAb^®, Cat. No. ZRB1761, is a recombinant Rabbit monoclonal antibody that specifically targets ZO-1 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Descripción de destino

Tight junction protein ZO-1 (UniProt: Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 (also known as ZO1) gene (Gene ID: 7082) in human. TJP1, TJP2, and TJP3 (ZO-1, ZO-2, and ZO-3) are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. ZO-1 is a homodimeric peripheral membrane protein that can also form heterodimers with TJP2 and TJP2 via its PDZ2 domain (aa 296-264). ZO-1 is reported to be essential for lumenogenesis and for efficient epithelial polarization and barrier formation. It also plays a role in podosome formation and associated functions, thus regulating cell adhesion and matrix remodeling. At podosome sites, it is predominantly localized in the ring structure surrounding the actin core. Two isoforms of ZO-1 have been described. The long isoform (-isoform) is found in most epithelial junction and the short isoform that lacks amino acids 922-1001 is found both in epithelial cells and in epithelial junctions of renal glomeruli and Sertoli cells of seminiferous tubules. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Forma física

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Información legal

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable