ZRB1546
Anti-Phospho-AKT1-(Ser473) Antibody, clone 1O14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Sinónimos:
RAC-alpha serine/threonine-protein kinase;EC:2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;Akt alpha
About This Item
Productos recomendados
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1O14, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from NIH3T3 cells treated with platelet derived growth factor (PDGF).
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected AKT1 in lysate from NIH3T3 cells serum starved overnight and treated with PDGF (100 ng/mL; 20 min).
Tested applications
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected AKT1 in lysate from HEK293 cells serum starved overnight and treated with Insulin (500 nM; 10 min).
Peptide Inhibition Assay: Target band detection in a lysate from HEK293 cells treated with insulin was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Affinity Binding Assay: A representative lot of this antibody bound AKT1 peptide with a KD of 1.1 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Phospho-AKT1-(Ser473) in human breast cancer and human melanoma tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
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Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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