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T3068

Sigma-Aldrich

Thrombin generation chromogenic substrate

≥90% (HPLC), solid

Sinónimos:

β-Ala-Gly-Arg-p-nitroanilide diacetate

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About This Item

Fórmula empírica (notación de Hill):
C21H34N8O9
Peso molecular:
542.54
MDL number:
MFCD03453625
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

synthetic (organic)

Quality Level

200

assay

≥90% (HPLC)

form

solid

technique(s)

activity assay: suitable

storage temp.

2-8°C

Amino Acid Sequence

Ala-Gly-Arg-pNA

Application

Thrombin generation chromogenic substrate can be used for chromogenic assay developed through aptamer affinity capture and a subsequent enzyme reaction for detecting the thrombin in dilute human serum.

Biochem/physiol Actions

Thrombin generation chromogenic substrate also known as beta-Ala-Gly-Arg para-nitroanilide, is proteolytically cleaved by human thrombin and produces beta-Ala-Gly-Arg and p-nitroanilide. The release of p-nitroanilide is quatified for assesing the anti-thrombin activity.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Qiang Zhao et al.
Biosensors & bioelectronics, 34(1), 232-237 (2012-03-06)
A simple chromogenic assay for human alpha thrombin is developed through aptamer affinity capture and a subsequent enzyme reaction. Thrombin is captured on the aptamer-modified magnetic beads, and catalyzes the conversion of chromogenic substrates to optically measured products. The measurement
Jan Van Den Abbeele et al.
PLoS pathogens, 6(6), e1000926-e1000926 (2010-06-10)
Tsetse flies are the notorious transmitters of African trypanosomiasis, a disease caused by the Trypanosoma parasite that affects humans and livestock on the African continent. Metacyclic infection rates in natural tsetse populations with Trypanosoma brucei, including the two human-pathogenic subspecies
D Prasa et al.
Thrombosis and haemostasis, 78(4), 1215-1220 (1997-11-19)
A series of inhibitors of factor Xa (FXa) were investigated using the thrombin generation assay to evaluate the potency and specificity needed to efficiently block thrombin generation in activated human plasma. By inhibiting FXa the generation of thrombin in plasma
Jingjing Wang et al.
Frontiers in microbiology, 12, 778309-778309 (2021-12-21)
African swine fever virus (ASFV) is a large double-stranded DNA virus and causes high mortality in swine. ASFV can be transmitted by biological vectors, including soft ticks in genus Ornithodoros but not hard ticks. However, the underlying mechanisms evolved in
Ning Luan et al.
Toxins, 11(12) (2019-12-11)
Elastase is a globular glycoprotein and belongs to the chymotrypsin family. It is involved in several inflammatory cascades on the basis of cleaving the important connective tissue protein elastin, and is strictly regulated to a balance by several endogenous inhibitors.

Protocolos

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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