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Sigma-Aldrich

Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

Sinónimos:

Reverse Transcriptase cDNA synthesis kit, eAMV-RT

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.55
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

200

uso

sufficient for 50 reactions
 kit sufficient for 50 reactions

Características

dNTPs included
hotstart: no

técnicas

RT-PCR: suitable

color

colorless

entrada

purified RNA

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.
Sigma′s Enhanced Avian RT First Strand Synthesis Kit also provides random nonamers (9-mers) and anchored oligo (dT)23 primers since gene-specific primers may not always be useful or possible. The anchored oligo has 23 thymidine residues and one G, C, or A residue (the anchor). The anchor ensures that the oligo (dT) primer binds at the very beginning of the message and that there is not a long region of the unusable sequence. With this kit, a dependable cDNA is generated that can be used for various downstream applications, including PCR.

Aplicación

Enhanced Avian First Strand Synthesis Kit has been used for cDNA synthesis, which is intended for use in a real-time reverse transcription-polymerase chain reaction (RT-PCR).[1][2]

Definición de unidad

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.

Información legal

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Solo componentes del kit

Referencia del producto
Descripción
  • Enhanced Avian Reverse Transcriptase 1000 U

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • Deoxynucleotide mix 50 μL
  • O4387Anchored oligo (dT)23 100 μLSDS
  • R7647Random nonamers 100 μLSDS
  • O4387Ribonuclease inhibitor 50 μLSDS
  • W1754PCR grade water 1.5 mLSDS

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SLCQ9453
SLCP5967
SLCN0255
SLCH8162
SLCH2897

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Oligo(dT)23, Anchored 70 μM in H2O

Sigma-Aldrich

O4387

Oligo(dT)23, Anchored

Random Nonamers for use as primers in cDNA synthesis, 50 μM in H2O

Sigma-Aldrich

R7647

Random Nonamers

Transcriptor Universal cDNA Master sufficient for 100 reactions, suitable for cDNA synthesis, suitable for RT-PCR, suitable for RT-qPCR

Roche

05893151001

Transcriptor Universal cDNA Master

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