Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

SRP3250

Sigma-Aldrich

Oncostatin-M from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinónimos:

OSM

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

24.4 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... OSM(289747)

General description

OSM (oncostatin M) is a polypeptide growth factor that belongs to the interleukin-6 cytokine family that includes interleukin-6, interleukin-11, leukemia inhibitory factor, ciliary neurotrophic factor, cardiotrophin-1, and novel neutrophin-1/B-cell-stimulating factor-3. In rats, this protein shows high expression in the late fetal and neonatal testis. It is also expressed at lower levels in the maturing and adult testis. It is also thought to be expressed in Leydig cells in developing testis.
Recombinant rat Oncostatin M is a 24.4kDa protein, containing 215 amino acid residues.

Biochem/physiol Actions

OSM (oncostatin M) functions as a ligand for two high affinity heterodimer receptors that share the signal transducing receptor gp130 (glycoprotein). The two receptors are- type I OSM receptor which is a dimer of the gp130 transducing receptor and the LIF (leukocyte inhibitory factor) receptor β-subunit, and the type II OSM receptor which contains a dimer of gp130 and the OSM receptor β-subunit. OSM is produced by neonatal Sertoli cells, where it induces Sertoli cell and gonocyte proliferation. It is responsible for the induction of spermatogenesis, and negates the effect of luteinizing hormone (LH) on Leydig cell progenitor formation, thus, acting as a regulator for this process. Study in rat shows that this protein stimulates the differentiation of OC15-5 (oval cells) into hepatocytes, and therefore, is thought to be key in liver regeneration by promoting oval cell differentiation into hepatocytes.

Sequence

MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Oncostatin M inhibits proliferation of rat oval cells, OC15-5, inducing differentiation into hepatocytes.
Okaya A et al
The American Journal of Pathology, 166(3), 709-719 (2005)
Oncostatin-M inhibits luteinizing hormone stimulated Leydig cell progenitor formation in vitro.
Teerds KJ et al
Reproductive Biology and Endocrinology, 5, 43-43 (2007)
Bruce, A.G., et al.
Cytokine Reference, 585-598 (2001)
T M Rose et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8641-8645 (1991-10-01)
Oncostatin M (OSM), a glycoprotein of Mr approximately 28,000 produced by activated monocyte and T-lymphocyte cell lines, was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. We have detected significant similarities
Zhen Wang et al.
Molecular medicine reports, 19(3), 1529-1542 (2018-12-29)
Psoriasis is a chronic inflammatory skin disease. Keratinocytes (KCs), as skin‑specific cells, serve an important role in the immunopathogenesis of psoriasis. In the present study, transcriptome data derived from psoriasis‑like KCs were used together with the reported transcriptome data from

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico