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Key Documents

SRP2011

Sigma-Aldrich

TFIIH, p62 subunit human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Sinónimos:

BTF2, TFB1, TFIIH

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

biological source

human

recombinant

expressed in E. coli

assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~64.4 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

750 μg/mL

color

clear colorless

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... GTF2H1(2965)

Biochem/physiol Actions

TFIIH, a multi-subunit complex is involved in several biological fundamental mechanisms of the cell: transcription, nucleotide excision repair and cell cycle regulation. p62 is one of the six subunits that constitutes the core of TFIIH. Analysis of the expression of the p62 gene reveals an over-expression in testis tissue. This subunit of TFIIH participates in a variety of protein-protein interactions. For example, Rb competes with TBP and p62 for binding to E2F thus repressing E2F-mediated trans-activation; herpes simplex virus VP16 and human p53 directly interact with the p62 subunit of TFIIH. In addition, TFIIH, via p62 phosphorylation is the major target for mitotic inactivation of transcription.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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W J Feaver et al.
The Journal of biological chemistry, 266(28), 19000-19005 (1991-10-05)
Heat treatment of yeast nuclear extracts abolished the capacity to initiate transcription at RNA polymerase II promoters. Activity was restored by the addition of both recombinant yeast TFIID and partially purified factor b, a yeast fraction shown previously to be
L Fischer et al.
Science (New York, N.Y.), 257(5075), 1392-1395 (1992-09-04)
Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide
O Flores et al.
The Journal of biological chemistry, 267(4), 2786-2793 (1992-02-05)
Two new factors required for transcription of class II genes have been identified. These factors, TFIIH and TFIIJ, were required together with the previously described general factors (TFIIA, TFIIB, TFIID, TFIIE, and TFIIF) and RNA polymerase II for transcription of

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