SRP0721

AKT1 (E17K), Active

PRECISIO^® Kinase, recombinant

• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• recombinant: expressed in baculovirus infected Sf9 cells
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 50-75 nmol/min·mg (radiometric)
• mol wt: ~85 kDa
• concentration: 0.1 μg/μL
• accession no.: NM_005163
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... AKT1(207)

Descripción

Application

Useful for kinase assay.

Biochem/physiol Actions

Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility ⁽¹⁾. AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias ⁽²⁾.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable