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Merck

SAB4200489

Sigma-Aldrich

Anti-Occludin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sinónimos:

Anti-OCLN

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

mol peso

antigen ~65 kDa

reactividad de especies

human, dog

concentración

~1.0 mg/mL

técnicas

immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon.
indirect immunofluorescence: 1-2 μg/mL using MDCK cells
western blot: 1.5-3.0 μg/mL using extracts of Caco-2 cells

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Descripción general

Occludin (OCLN) is an integral membrane protein, encoded by the gene mapped to human chromosome 5q13.2. OCLN has a molecular weight of about 65kDa. It possesses four transmembrane domains including a long COOH-terminal cytoplasmic domain, a short NH2-terminal cytoplasmic domain, two extracellular loops and one intracellular turn. It is specifically localized at tight junctions (TJ).

Inmunógeno

a synthetic peptide corresponding to a sequence at the C-terminal region of human occludin, conjugated to KLH. The corresponding sequence is highly conserved in rat (89% sequence identity) and in mouse (84% sequence identity) occludin.

Aplicación

Anti-Occludin (C-terminal) antibody produced in rabbit has been used in immunohistochemistry.

Acciones bioquímicas o fisiológicas

Occludin (OCLN), along with tight junction (TJ)-associated peripheral membrane proteins plays a vital role in the formation and regulation of TJ. The encoded protein facilitates the translocation of p85a (regulatory subunit of phosphoinositide 3-kinase) to TJs to regulate actin organization after oxidative stress. OCLN is also implicated in cell migration. Mutation in the gene leads to the development of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Visite la Librería de documentos

Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to Occludin
The Journal of Cell Biology, 141(7) (1998)
Recessive Mutations in the Gene Encoding the Tight Junction Protein Occludin Cause Band-like Calcification with Simplified Gyration and Polymicrogyria
Mary C, et al.
American Journal of Human Genetics (2010)
Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
Complex Phenotype of Mice Lacking Occludin, a Component of Tight Junction Strands
Mitinouri Saitou, et al
Molecular Biology of the Cell (2017)
Analyses of Gingival Adhesion Molecules in Periodontitis: Theoretical In Silico, Comparative In Vivo, and Explanatory In Vitro Models
Ulvi K, et al.
Journal of Periodontal Research (2015)
Splicing Diversity of the Human OCLN Gene and Its Biological Significance for Hepatitis C Virus Entry? ?
Indu Kohaar, et al.
Molecular and Cellular Biology (2010)

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