P8124
Pyoverdines
from Pseudomonas fluorescens, >90% (HPLC)
Sinónimos:
Pyoverdine Detection, Pyoverdine Protein, Siderophore Protein
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
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Productos recomendados
biological source
Pseudomonas fluorescens
Quality Level
assay
>90% (HPLC)
form
powder
solubility
H2O: ~10 mg/mL
shipped in
wet ice
storage temp.
−20°C
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Application
Pyoverdines from Pseudomonas fluorescens has been used :
- in tryptophan fluorescence quenching to determine its binding to neutrophil-gelatinase-associated lipocalin (NGAL)[1]
- to detect pyoverdine diffusion surrounding siderophore-conjugated monobactam (MB-1)-resistant colonies in chelexed, dialyzed Mueller-Hinton broth (CDMHB)[2]
- for synchronous fluorescence spectra of purified pyoverdine[3]
Biochem/physiol Actions
Pyoverdines help in promoting plant growth by chelating iron and rendering it unavailable for plant pathogens.[4] The ferri-pyoverdine complex acts as a signaling molecule inducing the production of secreted virulence factors in Pseudomonas aeruginosa.[5][6]
Pyoverdines, also called pseuobactins and pyoverdins, are fluorescent siderophores that have high affinity for iron (1032 M-1), and are synthesized by fluorescent pseudomonads under iron-deficient growth conditions. Pyoverdines were shown to prevent iron toxicity produced by iron overload in hepatocyte cultures and effectively scavenges the hydroxyl and peroxyl radicals. Pyoverdines are effective in acquiring iron from transferrin and lactoferrin. Pyoverdines are also involved in the suppression of pythium-induced damping-off of tomato and promotion of growth in some higher plants.
Pyoverdines, also called pseuobactins and pyoverdins, are fluorescent siderophores that have high affinity for iron, and are synthesized by fluorescent pseudomonads under iron-deficient growth conditions.
Physical form
Supplied as a mixture containing mainly the succinic acid (MW=1161), 2-hydroxy glutaramide (MW=1190), and succinamide (MW=1160) forms of pyoverdines.
Related product
Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Los clientes también vieron
Purification of Pyoverdines of Pseudomonas fluorescens 2-79 by Copper-Chelate Chromatography.
Xiao R and Kisaalita WS
Applied and Environmental Microbiology, 61(11), 3769-3774 (1995)
Correction: Multicolor Whole-Cell Bacterial Sensing Using a Synchronous Fluorescence Spectroscopy-Based Approach.
PLoS ONE (2015)
Pyoverdine, the Major Siderophore in Pseudomonas aeruginosa, Evades NGAL Recognition.
Peek ME
Interdisciplinary perspectives on infectious diseases, 843509-843509 (2012)
Biological activity of secondary metabolites produced by a strain of Pseudomonas fluorescens.
Boruah HP and Kumar BS
Folia Microbiologica, 47(4), 359-363 (2002)
Adaptation-based resistance to siderophore-conjugated antibacterial agents by Pseudomonas aeruginosa.
Tomaras AP
Antimicrobial Agents and Chemotherapy, 57(9), 4197-4207 (2013)
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