The product is provided as a 0.01% sterile filtered solution in water. When coating slides with this solution, it is recommended to incubate for 5 minutes and allow the slides to dry at room temperature.
P4707
Poly-ʟ-Lysine Hydrobromide
synthetic, liquid, 0.01%, suitable for cell culture
Sinónimos:
Disolución de PLL
About This Item
Productos recomendados
Nombre del producto
Poli-L-lisina solution, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
origen biológico
synthetic
Nivel de calidad
esterilidad
sterile-filtered
Línea del producto
BioReagent
Formulario
liquid
mol peso
70—150 kDa
envase
pkg of 50 mL
concentración
0.01%
técnicas
cell culture | mammalian: suitable
cobertura de la superficie
4 μg/cm2
impurezas
endotoxin, tested
solubilidad
water: soluble
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
cadena SMILES
N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
InChI
1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
Clave InChI
WBSCNDJQPKSPII-KKUMJFAQSA-N
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Descripción general
Aplicación
- Se han utilizado cubreobjetos de vidrio recubiertos con poli-L-lisina para montar muestras para el microscopio electrónico de barrido.[1]
- Se utilizaron pocillos recubiertos con poli-L-lisina para atrapar células PC-12 en los electrodos.[2]
- Se utilizaron portaobjetos de vidrio borosilicato recubiertos con poli-L-lisina para la siembra de células transducidas con vectores víricos.[3]
Acciones bioquímicas o fisiológicas
Componentes
Nota de preparación
Producto relacionado
producto comparable
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Protocolos
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
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When coating coverslips with item P4707, is it necessary to wash the reagent off after 5 minutes, or is there a different recommended procedure?
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Is the product P4707 suitable for coating glassware for cell culture? Also, what is the difference between Poly L and Poly D lysine?
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Yes, the product P4707 is suitable for coating glassware for cell culture. The difference between Poly L and Poly D lysine lies in their stereoisomeric properties. Poly-L-Lysine is a charge enhancer and can be used for coating many surfaces, including tissue culture plasticware and glassware. However, certain cells can digest poly-L-lysine, in which case the poly-D-lysine, such as P7405, should be used. This product can also be used to coat tissue culture plasticware and glassware.
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How many times can it be reused?
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The number of times that this product may be re-applied has not been determined. This would be dependent on several factors, such as surface area, material type, surface pretreatment, and incubation period. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/244/731/p4707pis.pdfHelpful?
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What is the molecular weight of product P4707?
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Poly-L-lysine solution is 70-150 kDa molecular weight.
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Should I use Poly-L-Lysine or Poly-D-Lysine?
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Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.
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What is the concentration of Product P4707, Poly-L-Lysine solution?
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This product is supplied as a 0.01% w/v solution.
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What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat tissue culture plasticware?
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A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.
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What molecular weight Poly-L-Lysine product should I choose?
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The lower molecular weight poly-L-lysine (30-70 kDa) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kDa) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kDa.
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What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat slides?
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For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.
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How can I sterilize slides coated with Product P4707, Poly-L-Lysine solution?
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Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.
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