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Key Documents

P4505

Sigma-Aldrich

Protamine sulfate

powder

Sinónimos:

Clupeine

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About This Item

Número de CAS:
9007-31-2
MDL number:
MFCD00132091
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Protamine sulfate salt from herring, Grade III, histone, free(Millon test)

biological source

fish (herring)

Quality Level

200

type

Grade III

form

powder

impurities

histone, free (Millon test)

solubility

6 M HCl: 25 mg/mL, clear, colorless to very faintly yellow

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Application

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Biochem/physiol Actions

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Moyra Lawrence et al.
Science advances, 8(7), eabj8618-eabj8618 (2022-02-17)
Platelet deficiency, known as thrombocytopenia, can cause hemorrhage and is treated with platelet transfusions. We developed a system for the production of platelet precursor cells, megakaryocytes, from pluripotent stem cells. These cultures can be maintained for >100 days, implying culture
A Bonincontro et al.
Physics in medicine and biology, 34(5), 609-616 (1989-05-01)
The complex dielectric constant of arginine and protamine from herring sperm (clupeine) and their complexes with herring sperm DNA was measured at 10 GHz in the temperature range -20 to +45 degrees C by a microwave cavity perturbation method. The
Lisanne T Laagland et al.
JOR spine, 5(3), e1219-e1219 (2022-10-08)
Repopulating the degenerated intervertebral disc (IVD) with tissue-specific nucleus pulposus cells (NPCs) has already been shown to promote regeneration in various species. Yet the applicability of NPCs as cell-based therapy has been hampered by the low cell numbers that can
L Willmitzer et al.
Biophysics of structure and mechanism, 6(2), 95-110 (1980-01-01)
The thermodynamics of protamine-DNA interation was investigated with clupeine Z from herring labeled at its amino terminus with fluorescein. The ionic strength dependence, the influence of protamine phosphorylation, of the native DNA conformation, using native and heat-denatured DNA, and of
Chengming Liu et al.
BMC medicine, 20(1), 187-187 (2022-05-14)
Cytokines have been reported to alter the response to immune checkpoint inhibitors (ICIs) in patients with the tumor in accordance with their plasma concentrations. Here, we aimed to identify the key cytokines which influenced the responses and stimulated resistance to
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