O4264

Human Osteopontin

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Sinónimos: Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• Número MDL: MFCD03100698
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• Nombre del producto: Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture
• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in NSO cells
• esterilidad: sterile
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: lyophilized powder
• mol peso: 60-65 kDa by SDS-PAGE (reducing)
• envase: pkg of 50 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin, tested
• Nº de acceso UniProt: P10451
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... SPP1(6696)

Descripción

Descripción general

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

Aplicación

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

Acciones bioquímicas o fisiológicas

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Nota de análisis

Bioactivity Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is is 0.100-0.600 ug/mL.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)