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Merck

N6658

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

Ham’s F-12

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75

Nombre del producto

Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Nivel de calidad

esterilidad

sterile-filtered

Formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.

Aplicación

Nutrient Mixture F-12 Ham has been used:
  • to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
  • to culture LAN-1 cells
  • to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
  • with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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