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M8787

Sigma-Aldrich

Magnesium chloride solution

PCR Reagent, 25 mM MgCI2 solution for PCR

Sinónimos:

PCR optimization

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About This Item

Fórmula lineal:
MgCl2
Número de CAS:
7786-30-3
Peso molecular:
95.21
Número MDL:
MFCD00011106
Código UNSPSC:
12352302
ID de la sustancia en PubChem:
57654422
NACRES:
NA.52
grado:
PCR Reagent
Formulario:
liquid

grado

PCR Reagent

Nivel de calidad

200
300

Formulario

liquid

envase

vial of 1.5 mL

concentración

25 mM±1 mM

técnicas

PCR: suitable

color

colorless

aplicaciones

agriculture
diagnostic assay manufacturing

actividad extraña

DNase, RNase, none detected

temp. de almacenamiento

2-8°C

cadena SMILES

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

Clave InChI

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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Descripción general

Magnesium chloride solution is suitable for the optimization of polymerase chain reactions (PCR). Magnesium chloride acts as a source of magnesium ions for polymerase chain reactions (PCR). It influences the primer-template annealing temperature, fidelity, specificity, and yield. Excess amounts of magnesium can cause an increase in nonspecific products, while lack of magnesium can lead to reduced yield.

Aplicación

Magnesium chloride solution has been used as a component of the:

  • amplification mixture for polymerase chain reaction (PCR)
  • amplification mixture in an inter simple sequence repeats (ISSR)-PCR
  • reaction buffer in 16S-23S rRNA gene internal transcribed spacer (ITS) PCR amplification and restriction analysis (ITS-PCR-RFLP)
  • PCR master mix for quantitative reverse transcription-polymerase chain reaction (qRT PCR) to amplify reversely transcribed cDNA
  • reaction mix for RT-PCR to assess the gene expression

Idoneidad

Suitable for optimization of polymerase chain reactions.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000308203
0000375058
0000287205
0000287361
0000287205

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Molecular characterization of Fusarium oxysporum f. sp. cubense isolates from banana
Das A, et al.
Pest Management in Horticultural Ecosystems , 18(2), 171-178 (2012)
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American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
Adewumi G A, et al.
Food Biotechnology, 28(3), 250-268 (2014)
Benjamin Dombert et al.
PloS one, 9(10), e110846-e110846 (2014-10-23)
Spinal muscular atrophy (SMA) is caused by deficiency of the ubiquitously expressed survival motoneuron (SMN) protein. SMN is crucial component of a complex for the assembly of spliceosomal small nuclear ribonucleoprotein (snRNP) particles. Other cellular functions of SMN are less
Fernando Cartón-García et al.
Scientific reports, 5, 12312-12312 (2015-07-24)
Inherited MYO5B mutations have recently been associated with microvillus inclusion disease (MVID), an autosomal recessive syndrome characterized by intractable, life-threatening, watery diarrhea appearing shortly after birth. Characterization of the molecular mechanisms underlying this disease and development of novel therapeutic approaches
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