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Merck

L8275

Sigma-Aldrich

Lectin from Galanthus nivalis (snowdrop)

lyophilized powder

Sinónimos:

Galanthus nivalis lectin, GNL

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.32

Formulario

lyophilized powder

Nivel de calidad

potencia

<20 μg per mL agglutination activity

composición

Protein, ≥90% Lowry

impurezas

salt, essentially free

temp. de almacenamiento

2-8°C

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Descripción general

Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.

Aplicación

Lectin from Galanthus nivalis (snowdrop) has been used:
  • to coat ELISA plates
  • as reverse transcriptase inhibitor
  • in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses

Acciones bioquímicas o fisiológicas

GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.
Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.

Nota de preparación

Purified by affinity chromatography according to Kaku.

Nota de análisis

Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Jacklyn Johnson et al.
Journal of virology, 94(9) (2020-02-28)
Semen is the primary transmission vehicle for various pathogenic viruses. Initial steps of transmission, including cell attachment and entry, likely occur in the presence of semen. However, the unstable nature of human seminal plasma and its toxic effects on cells
Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion
Hogervorst PAM, et al.
Journal of Insect Physiology, 52, 614-624 (2006)
Snowdrop lectin.
H Kaku et al.
Methods in enzymology, 179, 327-331 (1989-01-01)
A model for the hepatitis C virus envelope glycoprotein E2
Yagnik AT, et al.
Proteins: Structure, Function, and Bioinformatics, 40, 355-366 (2000)
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Hepatitis C virus (HCV) chronically infects 70 million people worldwide with an estimated annual disease-related mortality of 400,000. A vaccine could prevent spread of this pervasive human pathogen, but has proven difficult to develop, partly due to neutralizing antibody evasion

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