I9018
Monoclonal Anti-Involucrin antibody produced in mouse
clone SY5, ascites fluid
Sinónimos:
Anti-Involucrin
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About This Item
Productos recomendados
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SY5, monoclonal
contains
15 mM sodium azide
species reactivity
canine, gorilla, owl monkey, pig, human
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... IVL(3713)
General description
Involucrin is a soluble precursor protein that regulates terminal differentiation in keratinocytes. This protein is initially present in the cytosol component of keratinocytes and later associates with membrane proteins. Involucrin is expressed in squamous cell cancer and its expression is down-regulated by HPV 16 oncoproteins . Monoclonal Anti-Involucrin is specific for involucrin in primate and non-primate mammals. In cultured human keratinocytes, the antibody stains a 120 kDa band by immunoblot under denaturing-reducing conditions. However, in other cells the antibody binds to involucrin protein in varied sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approx. 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet at approx 105 kDa in pig. The antibody does not bind to mouse epidermis; hence the antibody can be used to study human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies .
Specificity
The antibody detects involucrin in a range of primate and nonprimate mammals in a variety of techniques. In immunoblotting of cultured human keratinocytes, the antibody stains a 120 kDa band under denaturing and reducing conditions. However, in other systems, the antibody stains the involucrin protein in a variety of sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approximately 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of ~105 kDa in pig. The antibody does not react with mouse epidermis, permitting use in studies of human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies.
Immunogen
human involucrin.
Application
Monoclonal Anti-Involucrin antibody is suitable for use in immunofluorescence at 1:100 dilutions using keratinocytes or for indirect immunofluorescence using methanol fixed mouse embryonic fibroblasts-keratinocytes co-cultures . The antibody may also be used for western blot (1:1000) and immunohistochemical analysis . Additionally, the product can be useful for indirect ELISA, immunoblotting, immunoprecipitation and immunocytochemistry.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Jun Dai et al.
PloS one, 8(7), e70392-e70392 (2013-08-08)
RORα is a retinoid-related orphan nuclear receptor that regulates inflammation, lipid metabolism, and cellular differentiation of several non-epithelial tissues. In spite of its high expression in skin epithelium, its functions in this tissue remain unclear. Using gain- and loss-of-function approaches
M N Ruesch et al.
Journal of virology, 72(6), 5016-5024 (1998-05-30)
The life cycle of the papillomaviruses is closely linked to host cell differentiation, as demonstrated by the fact that amplification of viral DNA and transcription of late genes occur only in the suprabasal cells of a differentiated epithelium. Previous studies
Z Suo et al.
Histopathology, 23(1), 45-54 (1993-07-01)
The expression of cytokeratins (CK) 1, 4, 5/6, 8, 13, 18, 19 and 20 and involucrin in 42 cases of squamous cell carcinomas from various locations was examined. The tumours expressed CK5/6 in 55%, CK8 in 76%, CK13 in 43%
Joan Manils et al.
Oncotarget, 6(26), 22375-22396 (2015-06-20)
TREX2 is a 3'-DNA exonuclease specifically expressed in keratinocytes. Here, we investigated the relevance and mechanisms of TREX2 in ultraviolet (UV)-induced skin carcinogenesis. TREX2 expression was up-regulated by chronic UV exposure whereas it was de-regulated or lost in human squamous
T Klymenko et al.
Journal of virology (2017-10-13)
The human papillomavirus (HPV) replication cycle is tightly linked to epithelial cell differentiation. To examine HPV-associated changes in the keratinocyte transcriptome, RNAs isolated from undifferentiated and differentiated cell populations of normal, spontaneously immortalised, keratinocytes (NIKS), and NIKS stably transfected with
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