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Merck

I5154

Sigma-Aldrich

IgG1, Kappa from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

Sinónimos:

Human IgG1-κ

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

human

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

assay

>95% (SDS-PAGE)

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass
Human myeloma IgG1, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

Application

The purified IgG1, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, κ was injected into mice (500 μg) to assess the T cell and B cell responses and also used for flow cytometry in CHO cells.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0.

Analysis Note

Identity is determined by immunoelectrophoresis and indirect ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yann Gallais et al.
Immunology and cell biology, 95(3), 306-315 (2016-10-08)
Patients treated with therapeutic biological products (BP) frequently develop anti-drug antibodies (ADA) with potential neutralizing capacities leading to loss of clinical response or serious side effects. BP aggregates have been suggested to promote immunogenicity, thus enhancing ADA production. Dendritic cells
Bas Cornelis Jansen et al.
PloS one, 13(7), e0200280-e0200280 (2018-07-07)
High-performance liquid chromatography (HPLC) is widely used for absolute quantitation. The advent of new columns and HPLC technology has enabled higher sample throughput, and hence, larger scale studies that perform quantitation on different sample types (e.g. healthy controls vs. patients
Taehong Kwon et al.
Analytical chemistry, 92(7), 5267-5275 (2020-03-14)
We demonstrate a new micro/nanofluidic system for continuous and automatic monitoring of protein product size and quantity directly from the culture supernatant during a high-cell-concentration CHO cell perfusion culture. A microfluidic device enables clog-free cell retention for a bench-scale (350
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Juan Manuel García-Martínez et al.
Molecular cancer therapeutics, 20(1), 96-108 (2020-10-11)
Activation of TRAILR2 has emerged as an important therapeutic concept in cancer treatment. TRAILR2 agonistic molecules have only had limited clinical success, to date, due either to lack of efficacy or hepatotoxicity. BI 905711 is a novel tetravalent bispecific antibody

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