H9289

Anti-Histone H3 (N-terminal) antibody produced in mouse

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Histone H3 Detection, Histone H3 Detection Antibody, Rabbit Anti-Histone H3
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~17 kDa
• reactividad de especies: human
• validación mejorada: independent; Learn more about Antibody Enhanced Validation
• técnicas: indirect immunofluorescence: 2-4 μg/mL using A431 cells.; western blot: 0.2-0.5 μg/mL using PC12 cell lysate and A431 cell lysate.
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HIST1H3A(8350)

Descripción

Descripción general

Anti-Histone H3 (N-terminal) specifically recognizes human histone H3 (~17kDa). Histone H3/ histone cluster 1 H3 family member a (HIST1H3A) is a core member of the histone family. HIST1H3A is encoded by the gene mapped to human chromosome 6p22.2. The encoded protein is composed of ~136 amino acid.

Inmunógeno

synthetic peptide corresponding to amino acids 1-20 of human histone H3, conjugated to KLH.

Aplicación

Anti-histone H3 (N-terminal) antibody produced in rabbit has been used in western blotting and native chromatin immunoprecipitation assay.[1]

Acciones bioquímicas o fisiológicas

Histones acts as structural scaffolds for DNA packaging. Histone H3 plays an important role in replication and DNA repair. Histone H3 is heavily post-translationally modified than the other members of histone family. Mutations in the gene leads to cerebellar high-grade gliomas.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Otras notas

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)