H6916

Macrophage Colony-Stimulating Factor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Sinónimos: MCSF
• MDL number: MFCD00166591
• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• product name: Macrophage Colony-Stimulating Factor human, M-CSF, recombinant, expressed in HEK 293 cells, suitable for cell culture
• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤4 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 35-40 kDa (glycosylated)
• packaging: pkg of 10 μg
• manufacturer/tradename: HumanZyme
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P09603
• storage temp.: −20°C
• Gene Information: human ... CSF1(1435)

Descripción

General description

M-CSF is encoded by the gene mapped to human chromosome 1p13.3. It is a glycosylated polypeptide, which exists as a homodimer. M-CSF is produced by macrophage, fibroblasts, lymphocyte and endothelial cells.

Biochem/physiol Actions

Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferation and differentiation of monocyte/macrophage-lineage cells. It also boosts antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages. In addition, human M-CSF inhibits bone resorption by osteoclasts. It might also play a crucial role in atherosclerosis. Overexpression of the gene has been observed in Paget′s disease of bone (PDB).

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid Leukemia indicator cell line).

Citation

1. Mazur, E.M., and Cohen, J.L., Clin. Pharmacol. Ther., 46, 250 (1989).
2. Morstyn, G., and Burgess, A.W., Cancer Res., 48, 5624 (1988).
3. Metcalf, D., Blood, 67, 257 (1986).
4. Mufson, R.A., et al., Cell. Immunol., 119, 182 (1989).
5. Hattersley, G., et al., J. Cell Physiol., 137, 199 (1988).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable