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Merck

H6136

SAFC

Hanks′ Balanced Salts

Without sodium bicarbonate, powder, suitable for cell culture

Fabricación farmacéutica

Sinónimos:

Hanks′ Balanced Salt Solution

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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General description

Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).

Application

Hanks′ Balanced Salt has been used:

  • in the preparation of stock solution for culturing mice primary hepatocytes
  • to measure the mineralized nodule formation in human osteoblast cultures
  • in the preparation of embryo buffer to collect zebrafish eggs

Quantity

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Behavioral profiling of zebrafish embryos exposed to a panel of 60 water-soluble compounds
Shaukat A, et al.
Behavioural Brain Research, 228, 272-283 (2012)
Osteoblast response to porous titanium and biomimetic surface: In vitro analysis
Renata F, et al.
Materials Science and Engineering, C, 52, 194-203 (2015)
Anish Deshpande et al.
Biomolecules, 10(3) (2020-03-25)
Alpha7 nicotinic acetylcholine receptors (α7nAChRs) are interesting not only because of their physiological effects, but because this receptor requires chaperones to traffic to cell surfaces (measured by alpha-bungarotoxin [αBGT] binding). While knockout (KO) animals and antibodies that react across species
Jennifer A-L M Pirotte et al.
The Journal of experimental biology, 221(Pt 20) (2018-09-13)
Winged aphids are described as hosts of lesser quality for parasitoids because a part of their resources is used to produce wings and associated muscles during their development. Host lipid content is particularly important for parasitoid larvae as they lack
Tom A P Driedonks et al.
Journal of extracellular vesicles, 9(1), 1764213-1764213 (2020-09-19)
Major efforts are made to characterize the presence of microRNA (miRNA) and messenger RNA in blood plasma to discover novel disease-associated biomarkers. MiRNAs in plasma are associated to several types of macromolecular structures, including extracellular vesicles (EV), lipoprotein particles (LPP)

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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