F3777

Anti-actina, α-músculo liso monoclonal

clone 1A4, purified from hybridoma cell culture

• Sinónimos: SMA
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 200
• conjugate: FITC conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 1A4, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human tonsil or appendix
• isotype: IgG2a
• UniProt accession no.: P62736
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTA2(59) ; mouse ... Acta2(11475) ; rat ... Acta2(81633)

Descripción

General description

El anticuerpo (también conocido como anti-α-Sm-1) es específico para la isoforma α-actina de músculo liso. Reacciona de manera específica con la α-actina del músculo liso en análisis de inmunotransferencia y marca las células del músculo liso en cortes de tejido incluidas en parafina congeladas o fijadas con formalina.

α-smooth muscle actin, a smooth muscle cell-specific protein, is also known as aortic smooth muscle or a smooth muscle actin (α-SMA, SMactin, α-SM-actin, ASMA). It belongs to the actin family.It is predominantly found in cells of the smooth muscle lineages. It has been reported that α-smooth muscle actin was also found in certain non-muscle cells, in particular fibroblasts i.e. myofibroblasts. Actin, a conserved eukaryotic protein, is the major cytoskeletal component and is expressed as six different isoforms in mammals and birds. It is mapped to human chromosome 10q23.31.

The antibody reacts with normal and neoplastic, human vascular and visceral smooth muscle cells. It reacts with normal myoepithelial cells, pericytes, eye lens cells, hair follicle cells and certain stromal cells in the intestine, testis, lymphoid organs, liver, ovary and bone marrow. The antibody also reacts with stromal myofibroblasts in hypertrophic scars, and in neoplastic tissues. α-smooth muscle actin is transiently co-expressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It was also found in the ventricular conducting tract of adult mammalian heart.

Specificity

Monoclonal Anti-Actin, α-Smooth Muscle specifically recognizes the α-smooth muscle isoform of actin (42 kDa) by ELISA and immunoblotting. It does not react with the other major actin isoforms present in fibroblasts or epithelial cells (β and γ-cytoplasmic), striated muscle (α-sarcomeric), myocardium (α-myocardial), or γ-smooth muscle isoform.

Immunogen

Decapéptido N-terminal sintético de la α-actina de músculo liso.

Application

Se realizó inmunocitoquímica en células de músculo liso de aortas bovinas utilizando el anticuerpo monoclonal anti-ACTA2. Las células se cultivaron primero en cubreobjetos de vidrio y se fijaron en acetona al 50%/EtOH durante 10 minutos a 4 grados.

Se tiñeron inmunohistoquímicamente cortes incluidos en parafina de injertos de tejido testicular de rata con anticuerpos monoclonales anti-actina de músculo liso.
Se realizó análisis mediante IHC de tejido cardiaco de ratón teñido con x-gal utilizando el anticuerpo monoclonal primario anti-actina de músculo liso de ratón para identificar los miofibroblastos.

Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody is suitable for:

• immunohistochemical (IHC) staining of heart, lung, kidney, and liver of mice to examine the PHD2 deficiency in internal organs
• immunostaining to analyze vascular smooth muscle cells/pericytes development in platelet-derived growth factor (PDGFR) and PDGFR- β^-/- embryos
• immunofluorescent analysis of implanted cells, frozen tissue samples to study the similar levels of expression of SMaA and SM-MHC as established primary SMC lines
• western blots of lens epithelial cells to determine the distribution of α-SMA expression in lens epithelial cell
• Immunohistochemical examination of lesion morphology
• flow cytometry
• immunohistochemistry, proliferation and TUNEL (terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling) assays
• immunocytochemistry

Biochem/physiol Actions

Actin plays an important role in cell motility, structure and integrity. Smooth muscle actin-α (SMA)/ α2-smooth muscle actin (ACTA2) interacts with β-myosin heavy chain and helps in vascular smooth muscle cell contraction. The encoded protein modulates the expression of c-MET (tyrosine-protein kinase Met) and focal adhesion kinase (FAK) in human lung adenocarcinoma cells, which positively and selectively mediates tumor progression. Mutation in the gene expression leads to thoracic aortic aneurysms and dissections (TAAD).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Protect from prolonged exposure to light.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)