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Merck
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F2637

Sigma-Aldrich

Polisacarosa 400

BioXtra, for molecular biology, lyophilized powder

Sinónimos:

Polysucrose 400, Ficoll 400

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About This Item

Número de CAS:
26873-85-8
Número MDL:
MFCD00081599
Código UNSPSC:
12352200
NACRES:
NA.52

origen biológico

non-animal source

Nivel de calidad

200

grado

Molecular Biology
for molecular biology

Línea del producto

BioXtra

Formulario

lyophilized powder

actividad extraña

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

temp. de almacenamiento

room temp

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Descripción general

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Aplicación

Usado para separación de célula y aislamiento de orgánulos.
Suitable for
  • hybridization experiments to reduce non-specific binding of labeled probes to the membrane
  • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
  • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
  • producing density gradients to separate subcellular components
  • vitrification of cell and tissue samples.
  • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).

Características y beneficios

  • free from DNases, RNases, phosphatases and proteases
  • preserves functional and morphological integrity of the separated cells

Otras notas

Un polímero sintético y no iónico de la sucrosa.

Información legal

Ficoll is a registered trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000317778
0000317775
0000317776
0000305252
0000353416

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Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
Cristina Batlle et al.
Cell reports, 30(4), 1117-1128 (2020-01-30)
Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been
Bohan Zhang et al.
Life sciences, 258, 118145-118145 (2020-07-28)
Studies on diabetes mellitus have shown that the phosphoinositide 3-kinase (PI3K)/serine threonine kinase (AKT)/forkhead box protein O1 (FoxO1) signaling pathway can regulate insulin secretion by modulating the expression of pancreatic and duodenal homeobox-1 (PDX-1). Therefore, it was hypothesized that the
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