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Merck

F1177

Sigma-Aldrich

Fluorescein hyaluronic acid

>95%, powder

Sinónimos:

Hyaluronic Acid Fluorescein Conjugate

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About This Item

MDL number:
UNSPSC Code:
12352201
NACRES:
NA.25

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Quality Level

assay

>95%

form

powder

mol wt

~800,000 Da

color

orange

solubility

H2O: soluble 1 mg/mL

storage temp.

2-8°C

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form

powder

form

powder

form

powder

form

powder

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

200

Application

Fluorescein hyaluronic acid has been used in the fluorescence imaging of hyaluronic acid (HA) and dextran surface coated films[1]and patterned surfaces.[2] It has also been used in confocal fluorescence microscopy to localize hyaluronic acid in collagen/HA composite gels .[3]

Biochem/physiol Actions

Hyaluronan macromolecules (HAs) coupled to fluorescein amine is exploited to screen the hyaluronidase activity using fluorescence correlation spectroscopy (FCS) by measuring the quenching of fluorescein-labeled HA fragments.[4] It binds on cell surface of malignant mesothelioma derived from human tumors.[5]
Used to bind on cell surface of malignant mesothelioma derived from human tumors.

Other Notes

From cock′s comb or by fermentation.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Visite la Librería de documentos

Rafal Fudala et al.
Journal of photochemistry and photobiology. B, Biology, 106, 69-73 (2011-11-16)
In this report we propose a lifetime-based sensing (LBS) for the detection of hyaluronidase (HA-ase). First, we heavily label hyaluronan macromolecules (HAs) with fluorescein amine. The fluorescein labeled HA (HA-Fl) has a weak fluorescence and short fluorescence lifetime due to
Human macrophage adhesion on polysaccharide patterned surfaces
Tsai IY, et al.
Soft Matter, 7(7), 3599-3606 (2011)
Hyaluronan receptors are expressed on human malignant mesothelioma cells but not on normal mesothelial cells
Asplund T and Heldin P
Cancer research, 54(16), 4516-4523 (1994)
Hyaluronan and dextran modified tubes resist cellular activation with blood contact
Eckmann DM, et al.
Colloids and Surfaces. B, Biointerfaces, 108, 44-51 (2013)
Rheology and confocal reflectance microscopy as probes of mechanical properties and structure during collagen and collagen/hyaluronan self-assembly
Yang Yl and Kaufman LJ
Biophysical Journal, 96(4), 1566-1585 (2009)

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