D4635

2′-Deoxycytidine 5′-triphosphate disodium salt

≥95%

• Sinónimos: dCTP-Na₂
• Fórmula empírica (notación de Hill): C₉H₁₄N₃Na₂O₁₃P₃
• Número de CAS: 102783-51-7
• Peso molecular: 511.12
• MDL number: MFCD00084683
• UNSPSC Code: 41106305
• PubChem Substance ID: 24893889
• NACRES: NA.51

Propiedades

• Quality Level: 200
• assay: ≥95%
• form: powder
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• InChI key: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Descripción

General description

2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine.[1] It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).[1]

Application

2′-Deoxycytidine 5′-triphosphate disodium salt has been used:

• in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNA[2]
• to spike oligonucleotide sample for capillary electrophoresis (CE) analysis[3]
• as a component in adenosine 5′-monophosphate (AMP) luciferase assay[4]

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Biochem/physiol Actions

2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases[5] or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase.[6] It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.[7]

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable