8C9

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Mouse x Mouse Hybridoma
• envase: tube of 5 μg 99072805-DNA-5UG; pkg of vial of cells 99072805-1VL
• ImmunoDon: BALB/c mouse spleen,female
• Mieloma: NS-1
• Reactividad: Anti-CD36, human
• técnicas: cell culture | mammalian: suitable
• isotipo: IgM
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse x Mouse hybridoma

Descripción línea celular

The hybridoma line 8C9 was produced by immunizing a female BALB/c mouse i.p. with tissue from a human malignant fibrous histiocytoma once a week for 6 weeks. Mouse spleen cells were fused with myeloma NS-1 cells using PEG and HAT for selection . The 8C9 monoclonal antibody detects a unique epitope on the leukocyte differentiation antigen CD36 (platelet glycoprotein IV or III6) expressed by normal and neoplastic endothelial cells. Immunoreactivity with 8C9 was detected on capillary endothelial cells, adipocytes, monocytes, platelets and the human monocytoid cell line U-937 (Sigma Catalogue number. 85011440). The antibody did not react with erythroid cells, T cells, B cells or HUVECs (human umbilical vein endothelial cells). Immunoblot analysis showed that 8C9 bound to a 97-kDa membrane protein expressed by U-937 cells treated with phorbol ester. Comparative immunohistochemical studies using 8C9 and the monoclonal antibody OKM demonstrated epitopic heterogeneity of the CD36 antigen by normal and neoplastic endothelial cells.

Inmunógeno

Human malignant fibrous histiocytoma

Aplicación

Study of endothelial cells, CD36 expression analysis

Medio de cultivo

RPMI 1640 + 2mM glutamine + 10% FBS

Rutina de subcultivo

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Otras notas

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