B16 melanoma 4A5 Cell Line from mouse

94042254, mouse skin, Fibroblast-like

• UNSPSC Code: 41106514

Propiedades

• product name: B16 melanoma 4A5 Cell Line from mouse, Mouse melanoma, producing melanin, 94042254
• biological source: mouse skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast-like
• products: Melanin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse melanoma, melanin producing

Cell Line Description

Derived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.

Application

B16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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