L929 Cell Line from mouse

85011425, mouse adipose tissue, Fibroblast

• Sinónimos: Earles′s Cells, L Cells, NCTC clone 929 Cells
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: L929 Cell Line from mouse, mouse C3H/An connective tisue, 85011425
• origen biológico: mouse adipose tissue
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse C3H/An connective tissue

Descripción línea celular

Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.

Aplicación

Virus studies: PRV, VSV (Indiana), HSV

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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