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Merck

C8696

Sigma-Aldrich

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

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About This Item

Número de CAS:
Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

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form

lyophilized powder

Quality Level

specific activity

≥250 units/mg protein (E1%/280)

mol wt

~45 kDa

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CTSD(1509)

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SAB3501252SAB3501249SAB3501247
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

purified antibody (Protein A)

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.[1]

Application

Cathepsin D from human liver has been used:
  • in β-secretase activity assay[2]
  • for enzymatic degradation of amyloid β 1-42[3]
  • in microinjection of human foreskin fibroblasts[4]

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Other Notes

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

Unit Definition

One unit will produce an increase in A280 of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

Physical form

Powder containing sodium phosphate buffer salt.

inhibitor

Referencia del producto
Descripción
Precios

related product

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Visite la Librería de documentos

Mengjie Ma et al.
Molecules (Basel, Switzerland), 28(13) (2023-07-14)
In this study, cathepsin D was oxidized in vitro with different concentrations of H2O2, and the activity, structure, and extent of myofibrillar protein degradation by oxidized cathepsin D were evaluated. The sulfhydryl content of cathepsin D decreased to 9.20% after
Aspartic Proteinases Physiology and Pathology (1995)
Platelet membrane beta-secretase activity in mild cognitive impairment and conversion to dementia: a longitudinal study
McGuinness B, et al.
Journal of Alzheimer'S Disease, 49(4), 1095-1103 (2016)
Jeane do Nascimento Moraes et al.
The journal of venomous animals and toxins including tropical diseases, 28, e20220002-e20220002 (2022-11-22)
Cathepsin D (CatD) is a lysosomal proteolytic enzyme expressed in almost all tissues and organs. This protease is a multifunctional enzyme responsible for essential biological processes such as cell cycle regulation, differentiation, migration, tissue remodeling, neuronal growth, ovulation, and apoptosis.
Marie Maynadier et al.
Journal of controlled release : official journal of the Controlled Release Society, 171(2), 251-257 (2013-08-01)
Implication of the intracellular proteolytic activity of Cathepsin D (CathD), a lysosomal aspartyl-protease overexpressed in numerous solid tumors, has been evidenced on tumor growth. Its intracellular inhibition by potent inhibitors such as pepstatin constitutes a relevant but challenging molecular target.

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