C8104
Monoclonal Anti-Ceramide antibody produced in mouse
clone MID 15B4, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Ceramide Antibody, Ceramide Antibody - Monoclonal Anti-Ceramide antibody produced in mouse
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MID 15B4, monoclonal
form
buffered aqueous solution
technique(s)
immunohistochemistry (frozen sections): suitable
indirect ELISA: 1:10
isotype
IgM
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Ceramide is an endogenous lipid component of a novel biochemical pathway termed the sphingomyelin pathway. Ceramide is produced in response to cellular stimulation by hormones, cytokines and antigens. Ceramide synthesis is mediated either by the salvage pathway by the acylation of sphingosine or sphingolipids hydrolysis or by de novo pathway via dihydroceramide formation. Structurally, ceramides have a sphingoid base, a long-chain amino alcohol linked to fatty acid by an amide bond. Ceramide is generated by hydrolysis of sphingomyelin by sphingomyelinase.
Specificity
Mouse monoclonal clone MID 15B4 anti-Ceramide antibody recognizes free and bound ceramides.
Immunogen
ceramide conjugated to bovine serum albumin.
Application
Monoclonal Anti-Ceramide antibody produced in mouse has been used in:
- immunohistochemistry
- immunolabeling in electron microscopy
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- as a probe to determine the presence and roles of ceramide in sphingomyelin pathway signaling and the regulation of protein phosphorylation.
Biochem/physiol Actions
Ceramide appears to have a role in mediating biological responses in a wide variety of cell types. Ceramide metabolites such as sphingosine and sphingosine-1-phosphate have potent biological activities of their own. They are directly involved in the proliferation and differentiation of skin cells and regulate skin barrier functionality. Ceramide based analogs are potential anti-tumor agents and are regarded as tumor suppressor lipid. Mechanisms for ceramide action involve regulation of protein phosphorylation via stimulation of a serine/threonine protein phosphatase, a proline-directed kinase and possibly other direct and/or indirect targets. Ceramide is emerging as an intracellular messenger than mediates effects on terminal differentiation and cell proliferation as well as apoptosis or cell death and cell-cycle arrest. The interrelationship of ceramide actions with other bioactive lipids and systems represents an area of active research.
Physical form
Solution in phosphate buffered saline containing 0.5 M NaCl, 0.1% bovine serum albumin, and 0.09% sodium azide.
Preparation Note
Purified from ascites fluid by gel filtration using sephacryl S-300 resin.
Analysis Note
The antibody was characterized on ceramide covalently bound to myoglobin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Ignazio Restivo et al.
International journal of molecular sciences, 24(2) (2023-01-22)
Cell death program of red blood cells (RBCs), called eryptosis, is characterized by activation of caspases and scrambling of membrane phospholipids with externalization of phosphatidylserine (PS). Excessive eryptosis confers a procoagulant phenotype and is implicated in impairment of microcirculation and
Zi-Jun Sun et al.
JCI insight, 7(24) (2022-12-23)
Accumulating evidence suggests the pathogenic role of immunity and metabolism in diabetic kidney disease (DKD). Herein, we aimed to investigate the effect of complement factor B (CFB) on lipid metabolism in the development of DKD. We found that in patients
ELOVL4-mediated production of very long-chain ceramides stabilizes tight junctions and prevents diabetes-induced retinal vascular permeability
Kady NM, et al.
Diabetes, 67(4), 769-781 (2018)
Jormay Lim et al.
Communications biology, 3(1), 45-45 (2020-01-29)
Intraflagellar transport (IFT) proteins are essential for cilia assembly and function. IFT protein mutations lead to ciliopathies, which manifest as variable skeletal abnormalities. However, how IFT proteins regulate cell alignment during bone development is unknown. Here, we show that the
Matthias Corrotte et al.
eLife, 2, e00926-e00926 (2013-09-21)
Rapid repair of plasma membrane wounds is critical for cellular survival. Muscle fibers are particularly susceptible to injury, and defective sarcolemma resealing causes muscular dystrophy. Caveolae accumulate in dystrophic muscle fibers and caveolin and cavin mutations cause muscle pathology, but
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico