A1908

Asialofetuin from fetal calf serum

Type II (Sigma designation)

• Número MDL: MFCD00130561
• Código UNSPSC: 12352202
• NACRES: NA.61

Propiedades

• origen biológico: bovine
• Nivel de calidad: 200
• tpo: Type II (Sigma designation)
• Formulario: powder
• técnicas: activity assay: suitable; inhibition assay: suitable
• impurezas: salt, essentially free; ≤1.0% N-acetylneuraminic acid
• solubilidad: H₂O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

Asialofetuin from fetal calf serum is suitable for use:

• in the assay of the lactose binding activity of the galectin-7 by immunodetection of in situ binding activity to asialofetuin immobilized on nitrocellulose
• in the inhibition of zona pellucida hardening of mouse oocytes
• as an acceptor in sialyltransferase enzyme activity assay

Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Acciones bioquímicas o fisiológicas

Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.

Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.

Nota de preparación

Enzymatic cleavage using neuraminidase

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)