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Key Documents

83850

Sigma-Aldrich

Ribonucleic acid from Torula utilis

Mr 5000-8000

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About This Item

MDL number:
UNSPSC Code:
12352106
NACRES:
NA.51

grade

for molecular biology

Quality Level

form

solid

mol wt

Mr 5000-8000

impurities

≤10% water

solubility

0.1 M acetate buffer pH 5.0: soluble 10 mg/mL

shipped in

wet ice

storage temp.

−20°C

General description

RNA from yeast is used in molecular biology laboratories as a carrier during nucleic acid purifications. When used at 10-20 μg/ml, the tRNA acts as a potent co-precipitant that allows for maximal RNA recovery (when working with limited samples).

Application

Ribonucleic acid from Torula utilis was used as a standard in ribonuclease activity assay of certain ribonuclease family members. It was also used as a standard to determine RNA concentration by orcinol assay.
Suitable for use in nucleic acid purifications.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Los clientes también vieron

Debi Ranjan Tripathy et al.
Analytical biochemistry, 437(2), 126-129 (2013-03-19)
The ribonuclease (RNase) activity of ribonucleases has been assayed by observing the change in fluorescence intensity of ethidium bromide on binding with yeast RNA. The binding of EtBr with RNA was monitored via UV-vis and fluorimetric methods. The degradation of
Russel J Lander et al.
Biotechnology and bioengineering, 79(7), 776-784 (2002-09-05)
Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phase chromatography (RPC), and size

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