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56759

Sigma-Aldrich

Atto 425

BioReagent, suitable for fluorescence

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About This Item

Fórmula empírica (notación de Hill):
C22H27NO6
Número de CAS:
652966-03-5
Peso molecular:
401.45
UNSPSC Code:
12352108
PubChem Substance ID:
329758237
NACRES:
NA.32

product line

BioReagent

Quality Level

100

assay

≥90% (HPLC)

form

(Report result)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, >90%
425 nm, >90%

fluorescence

λex 440 nm; λem 484 nm

λ

in PBS, pH 7.4
 in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 423-429 nm Amax

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

SMILES string

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(O)=O)c3cc2OC1=O

InChI

1S/C22H27NO6/c1-5-28-20(26)16-10-14-9-15-13(2)12-22(3,4)23(8-6-7-19(24)25)17(15)11-18(14)29-21(16)27/h9-11,13H,5-8,12H2,1-4H3,(H,24,25)

InChI key

WNDDWSAHNYBXKY-UHFFFAOYSA-N

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 425 is suitable for preparation of fluorescence labeling reagents.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
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The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
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A new approach for the determination of the amyloid fibril - thioflavin T (ThT) binding parameters (the number of binding modes, stoichiometry, and binding constants of each mode) is proposed. This approach is based on the absorption spectroscopy determination of
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