19899

CHAPS 100 mM solution

• Sinónimos: CHAPS solution
• Fórmula empírica (notación de Hill): C₃₂H₅₈N₂O₇S
• Número de CAS: 75621-03-3
• Peso molecular: 614.88
• Número MDL: MFCD00012116
• Código UNSPSC: 12161902
• ID de la sustancia en PubChem: 57647807
• NACRES: NA.25

Propiedades

• descripción: zwitterionic
• Nivel de calidad: 100
• Formulario: liquid
• mol peso: 614.88 g/mol
• concentración: 100 mM in H₂O
• técnicas: protein quantification: suitable
• CMC: 8 mM
• temperatura de transición: cloud point >100 °C
• densidad: 1.01 g/mL at 20 °C
• temp. de almacenamiento: 2-8°C
• cadena SMILES: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• Clave InChI: UMCMPZBLKLEWAF-RFCNGIAKSA-N

Descripción

Descripción general

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.

Aplicación

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs) and liver tissue.

Acciones bioquímicas o fisiológicas

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves