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Merck

12106C

Sigma-Aldrich

Suero fetal bovino

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

Sinónimos:

FBS, FCS, Suero, sueros

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.71
origen biológico:
bovine fetus
origen:
USA origin
esterilidad:
sterile-filtered
Condiciones de envío:
dry ice
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

bovine fetus

Nivel de calidad

esterilidad

sterile-filtered

Línea del producto

FBS Premier

composición

hemoglobin, ≤20 mg/dL

origen

USA origin

técnicas

cell culture | mammalian: suitable

impurezas

≤10 EU/mL endotoxin

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

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Aplicación

Fetal Bovine Serum (FBS) provides many non-defined growth promoting and survival enhancing factors to cells in culture. FBS, heat inactivated may be used in place of regular FBS wherein the inactivation of the complement system is beneficial to cell growth or function. Heat-inactivated FBS is used to culture Leishmania sp.[1][2]

Nota de preparación

Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Nota de análisis

  • Analizada la presencia de endotoxinas y hemoglobina
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves


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Certificados de análisis (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer
    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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