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06966

Sigma-Aldrich

Atto 655-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Sinónimos:

Biotin-Atto 655

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About This Item

UNSPSC Code:
12352116
NACRES:
NA.32

product line

BioReagent

Quality Level

100

assay

≥90% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
655 nm

solubility

DMF: soluble
DMSO: soluble
acetonitrile: soluble

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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