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Merck

06966

Sigma-Aldrich

Atto 655-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Sinónimos:

Biotin-Atto 655

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About This Item

Código UNSPSC:
12352116
NACRES:
NA.32

Línea del producto

BioReagent

Nivel de calidad

Ensayo

≥90% (HPCE)

Formulario

powder

fabricante / nombre comercial

ATTO-TEC GmbH

transmitancia

254 nm
655 nm

solubilidad

DMF: soluble
DMSO: soluble
acetonitrile: soluble

fluorescencia

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

Aplicación

Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.

Información legal

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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