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03285

Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

Sinónimos:

Fluorescent Cell Counter

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About This Item

Código UNSPSC:
12352116
eCl@ss:
32161000
NACRES:
NA.32

Formulario

liquid

Nivel de calidad

100

uso

sufficient for 500 tests

temp. de almacenamiento

−20°C

Descripción general

Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.

Aplicación

Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.

Características y beneficios

Cell Counting Kit has the following advantages:

  • It doesn’t require any radioisotopes to function.
  • No solubilization procedure is needed.
  • Results are highly reproducible and accurate.

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
BCCL9020
BCCJ4198
BCCF8186
BCCD4653
BCCB4072

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Bin Zhou et al.
International journal of molecular medicine, 46(4), 1571-1581 (2020-09-19)
Osteoporosis is a systemic metabolic bone disease during which bone mass decreases and bone quality is reduced. Maintaining the bone formation capacity of osteoblasts is crucial for the treatment of osteoporosis. In the present study, bioinformatics analysis was performed on
Zhenhua Ding et al.
Medicine, 94(33), e1327-e1327 (2015-08-20)
Proteolipid protein 2 (PLP2) has been shown to be upregulated in several cancers, including breast cancer, hepatocellular carcinoma, osteosarcoma, and melanoma. PLP2 specifically binds to phosphatidylinositol 3 kinase to activate the protein kinase B pathway to enhance cell proliferation, adhesion
Jianmin Li et al.
Laboratory investigation; a journal of technical methods and pathology, 101(2), 142-154 (2020-09-30)
Epithelial-to-mesenchymal transition (EMT) of epithelium and airway epithelial cell proliferation disorder are key events in idiopathic pulmonary fibrosis (IPF) pathogenesis. During EMT, epithelial cell adhesion molecules (EpCAM, such as E-cadherin) are downregulated, cytokeratin cytoskeletal transforms into vimentin-based cytoskeleton, and the
Zhongyi Zhou et al.
Aging, 13(7), 9838-9858 (2021-03-22)
Colorectal cancer (CRC) is a prevalent malignancy worldwide. The development of genome sequencing technology has allowed the discovery that epigenetic regulation might play a critical role in CRC tumorigenesis. In the present study, we found that the long noncoding RNA
Nan Zhang et al.
Oncology letters, 12(6), 4589-4597 (2017-01-21)
MicroRNA (miR)-217 has been reported to participate in carcinogenesis and tumor progression in several cancers; however, its expression and biological functions in colorectal cancer (CRC) are still unclear. The present study demonstrated that miR-217 expression was significantly higher in matched
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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