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Merck

P4864

Sigma-Aldrich

Propidium iodide solution

≥94% purity, solution (1.0 mg/ml in water)

Sinónimos:

3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium iodide methiodide, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium, diiodide

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About This Item

Fórmula empírica (notación de Hill):
C27H34I2N4
Número de CAS:
Peso molecular:
668.39
Beilstein/REAXYS Number:
3843838
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Propidium iodide solution,

assay

≥94%

Quality Level

form

liquid

color

faint orange to very dark orange, and Faint Red to Very Dark Red and Faint Purple to Very Dark Purple and Orange-Red and Red-Orange

solubility

water: 1 mg/mL

εmax

5400-6400 at 491-495 nm in phosphate buffer

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

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General description

Propidium iodide solution is used to assess neuronal death.

Application

Propidium iodide solution has been used:
  • in staining DNA
  • in the viability staining method to study islet viability
  • to visualize F-actins and nuclei in mesenchymal stem cell sheets

Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

Related product

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Visite la Librería de documentos

Stroke E-Book: Pathophysiology Diagnosis and Management, 79-79 (2011)
Variation in human islet viability based on different membrane integrity stains
Barnett MJ, et al.
Cell Transplantation, 13(5), 481-488 (2004)
Direct intramyocardial injection of mesenchymal stem cell sheet fragments improves cardiac functions after infarction
Wang CC, et al.
Cardiovascular Research, 77(3), 515-524 (2007)
Paternal cyclophosphamide exposure induces the formation of functional micronuclei during the first zygotic division
Grenier L, et al.
PLoS ONE, 6(11), e27600-e27600 (2011)
Chung-Chi Wang et al.
Cardiovascular research, 77(3), 515-524 (2007-11-17)
Cell transplantation is a promising approach for patients with myocardial infarction. However, following injection, retention of the transplanted cells in the injected area remains a central issue, which can be deleterious to cell transplantation therapy. We hypothesized that the use

Artículos

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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