902020

Desthiobiotin-PEG3-Azide

• Sinónimos: (4S,5R)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]-5-methyl-2-oxo-4-imidazolidinehexanamide
• Fórmula empírica (notación de Hill): C₁₈H₃₄N₆O₅
• Número de CAS: 1951424-99-9
• Peso molecular: 414.50
• MDL number: MFCD28334534
• UNSPSC Code: 51171641
• NACRES: NA.22

Propiedades

• form: paste
• storage temp.: −20°C

Descripción

Application

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 10¹¹ vs. 10¹⁵ M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable