11585886001

Neuraminidase (Sialidase)

from Clostridium perfringens

• Sinónimos: Sialidase
• Comisión internacional de enzimas: 3.2.1.18 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: bacterial (Clostridium perfringens)
• Nivel de calidad: 100
• Formulario: lyophilized
• actividad específica: 100 U/mg; ~100 units/mg protein
• mol peso: 60 kDa
• envase: pkg of 5 U
• fabricante / nombre comercial: Roche
• pH óptimo: 5
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

approximately 100 U/mg protein at +37°C and pH 5.0 with N-acetyl-neuraminosyl-D-lactose as the substrate.

Especificidad

Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,3 >α2,8 = α2,6, determined on bonds in tri- and tetrasaccharides.

Aplicación

Neuraminidase (Sialidase) has been used to desialylate transferrin in order to study its isoforms in human serum.

Use Neuraminidase to hydrolyze terminal N- or 0-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate α2,3: > α2,6 = α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids.In contrast to the enzyme from Arthrobacter ureafaciens, neuraminidase from Clostridium perfringens hydrolyzes α2,3-linkages faster than α2,6-linkages. α2,8-bound sialic acids area cleaved with a similar velocity compared to α2,6-bound sialic acids.
Neuraminidase is used for:

• Virus receptor studies
• Studies on the interaction of lymphocytes with tumor cells
• Cell hybridizations
• Analysis of oligosaccharides
• Analysis of glycoproteins
• Analysis of glycolipids

Acciones bioquímicas o fisiológicas

Neuraminidase breaks α-ketosidic linkage between N-acetylneuraminic acid and the adjacent sugar residue.

Neuraminidase mediates apoptosis in the host cell before viral entry.

Nota de preparación

Stabilizers: The enzyme can be stabilized by bovine serum albumin (BSA).
Storage conditions (working solution): After reconstitution in double-dist. water or sample buffer, the enzyme is stable for several weeks, stored at 2 to 8 °C; for longer storage, freezing is recommended. A stock solution may be made (e.g., at c = 5 U/100 μl). The enzyme looses approx. 50% of its activity after incubation at 37 °C for 24 hours.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash