10838039001

Fibronectina

from human plasma

• Sinónimos: Fibronectin
• Número MDL: MFCD00131062
• Código UNSPSC: 12352207

Propiedades

• origen biológico: human plasma
• Nivel de calidad: 100
• esterilidad: non-sterile; 0.2 μm filtered
• Ensayo: 95% (SDS-PAGE)
• Formulario: lyophilized (clear, colorless solution after reconstitution)
• mol peso: 440 kDa
• envase: pkg of 5 mg
• fabricante / nombre comercial: Roche
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• técnicas: cell culture | mammalian: suitable
• Nº de acceso UniProt: P02751
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... FN1(2335)

Descripción

Descripción general

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.[1]

Especificidad

Active on most mammalian cells

Aplicación

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.[2][3][4]

Método de análisis

Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage)

Forma física

Lyophilizate (contains 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin), before lyophilization filtered through a 0.2 μm pore size membrane.

Nota de preparación

Working concentration: 5 μg/cm²
5 μg/cm² for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Reconstitución

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate for 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash