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Key Documents

MABF2079M

Sigma-Aldrich

Anti-CD8 alpha Antibody, clone 53-6.72

clone 53-6.72, from rat

Sinónimos:

T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

53-6.72, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG2aκ

NCBI accession no.

General description

T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the CD8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 alpha is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved to form the mature protein that has an extracellular domain (aa 28-196), a helical domain (aa 197-217), and a cytoplasmic tail (aa 218-247). CD8 is generally present as a heterodimer of alpha and beta chains linked by two disulfide bonds, however, it can also form homodimers. The CD8 alpha/alpha homodimer is expressed on a subset of gamma/delta TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 plays a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. CD8 acts as a co-receptor for the T cell receptor (TCR) and like the TCR, it binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets.

Specificity

Clone 53-6.72 is a rat monoclonal antibody that detects CD8 alpha chain in murine thymocytes.

Immunogen

Murine Thymic/splenic lymphocytes.

Application

Flow Cytometry Analysis: A representative lot detected CD8 alpha in Flow Cytometry applications (de Latour, R.P., et. al. (2010). Blood. 116(20):4175-84).

Functional Analysis: A representative lot detected CD8 alpha in Functional analysis applications (Daley, D., et. al. (2016). Cell. 166(6):1485-1499; Hannani, D., et. al. (2014). Cell Death Differ. 21(1):50-8).
This rat monoclonal Anti-CD8 alpha, clone 53-6.72, Cat. No. MABF2079, is a rat monoclonal antibody that detects CD8 alpha and has been tested for use in Flow Cytometry and functional studies.

Quality

Evaluated by Flow Cytometry in mouse thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CD8 alpha in one million mouse thymocytes.

Target description

27.46 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2


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