MABE288
Anti-PCNA Antibody, clone PC10
clone PC10, from mouse
Sinónimos:
Proliferating cell nuclear antigen, PCNA, Cyclin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PC10, monoclonal
species reactivity
human
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PCNA(5111)
General description
Proliferating cell nuclear antigen (PCNA) is a heterotrimeric nuclear protein that functions as a clamp on replicating DNA. In cases of DNA damage and failure of the DNA repair pathways, PCNA mediates translesion DNA synthesis, a DNA damage tolerance (DDT) pathway, which allows replication to progress in spite of large lesions to DNA which may be caused by replication stress, ionizing radiation, spontaneous DNA damage, among other factors. PCNA primarily recruits low-fidelity DNA polymerase enzymes that are capable of elongating DNA over lesion sites. PCNA is regulated by cycles of ubiquitination, depending on the DDT pathway involved: PCNA is monoubiquitinated by Rad6–Rad18; polyubiquitinated by the Mms2-Ubc13-Rad5; or sumoylated by the Ubc9-Siz1 complex.
Immunogen
Recombinant protein corresponding to human PCNA.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PCNA in human colon adenocarcinoma tissues and human tonsil tissues.
Immunofluorescence Analysis: A 1:1,500 dilution from a representative lot detected PCNA in human lymph nodes and in human colon adenocarcinoma cells.
Immunofluorescence Analysis: A 1:1,500 dilution from a representative lot detected PCNA in human lymph nodes and in human colon adenocarcinoma cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody) validated in WB, IHC, IF to detect PCNA also known as Proliferating cell nuclear antigen, PCNA, Cyclin.
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody recognized PCNA in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.01 µg/mL of this antibody recognized PCNA in 10 µg of HeLa cell lysate.
Target description
~29 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Loading controls in western blotting application.
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