MABE1799
Anti-Nrf2 Antibody, clone 103
clone 103, from rat
Sinónimos:
Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, like 2
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
103, monoclonal
species reactivity
rat, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
mouse ... Nfe2L2(18024)
General description
Nuclear factor erythroid 2-related factor 2 (UniProt: Q60795; also known as Nrf2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, like 2) is encoded by the Nfe2l2 (also known as Nrf-2, Nrf2) gene (Gene ID: 18024) in murine species. Nrf2 is a cap n collar basic-region leucine zipper transcription factor that is involved in adaptation of cells to oxidative stress and electrophiles. It bind to antioxidant response (ARE) elements in the promoter regions of target genes and induces a set of cytoprotective genes that are involved in the synthesis of reduced glutathione (GSH), repair of oxidized protein thiols, scavenging of reactive oxygen species, and drug metabolism. It is cytosolic under unstressed conditions, but translocates into the nucleus upon induction by electrophilic agents. Under normal unstressed conditions it is rapidly turned over in a Keap-1 dependent manner through Cul3-Rbx1 ubiquitination and proteasomal degradation. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates Nrf2 from its Keap-1 and promotes its translocation into the nucleus. It is widely expressed with highest expression in liver, skeletal muscle, luminal cells of the stomach and intestine, lining of the bronchi and alveoli, and in renal tubules; followed by heart, spleen, testis and brain. Nrf2 is constitutively upregulated in many tumors due to somatic mutations in the Keap1 or Nrf2 genes. Tumors overexpressing Nrf2 exhibit increased resistance to chemotherapeutic drugs and higher rates of proliferation. (Ref.: Hayes, JD., and Ashford, MLJ (2012). Cell Metab. 16(2): 139-141).
Specificity
Clone 103 detects Nrf2 in Keap-1 knockout cells. It targets an epitope within 179 amino acids in the internal region.
Immunogen
GST-tagged recombinant fragment corresponding to 179 amino acids from internal region of murine Nuclear factor erythroid 2-related factor 2.
Application
Anti-Nrf2, clone 103 , Cat. No. MABE1799, is a highly specific rat monoclonal antibody that targets Nuclear factor erythroid 2-related factor 2 and has been tested in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected Nrf2 in Immunohistochemistry applications (Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84).
Western Blotting Analysis: A representative lot detected Nrf2 in Western Blotting applications (Maruyama, A., et. al. (2008). Arch Biochem Biophys. 477(1):139-45; Kobayashi, E.H., et. al. (2016). Nat Commun. 7:11624; Taquchi, K., et. al. (2016). Toxicol Sci. 152(1):40-52; Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84; Taquchi, K., et. al. (2014). Mol Cell Biol. 34(5):900-13).
Western Blotting Analysis: A representative lot detected Nrf2 in Western Blotting applications (Maruyama, A., et. al. (2008). Arch Biochem Biophys. 477(1):139-45; Kobayashi, E.H., et. al. (2016). Nat Commun. 7:11624; Taquchi, K., et. al. (2016). Toxicol Sci. 152(1):40-52; Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84; Taquchi, K., et. al. (2014). Mol Cell Biol. 34(5):900-13).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nrf2 in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nrf2 in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.
Target description
~67 kDa observed; 66.90 kDa calculated Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Ye Tian et al.
PloS one, 18(1), e0279964-e0279964 (2023-01-07)
Sepsis associated encephalopathy (SAE) is a common but poorly understood complication during sepsis. Currently, there are no preventive or therapeutic agents available for this neurological disorder. The present study was designed to determine the potential protective effects of β-patchoulene (β-PAE)
Carmen Rodríguez-García et al.
Molecular nutrition & food research, 66(18), e2200082-e2200082 (2022-07-19)
To compare the effects of three high-fat diets (HFDs) based on coconut, sunflower, or extra virgin olive oils (EVOOs) on adipose tissue, metabolism, and inflammation. Mice are fed for 16 weeks on their respective HFD. HFD based on coconut oil produces
Greg A Timblin et al.
Nature metabolism, 3(5), 618-635 (2021-05-26)
Macrophages generate mitochondrial reactive oxygen species and mitochondrial reactive electrophilic species as antimicrobials during Toll-like receptor (TLR)-dependent inflammatory responses. Whether mitochondrial stress caused by these molecules impacts macrophage function is unknown. Here, we demonstrate that both pharmacologically driven and lipopolysaccharide
Yuening Liu et al.
The Journal of biological chemistry, 297(1), 100850-100850 (2021-06-05)
Reperfusion therapy, the standard treatment for acute myocardial infarction, can trigger necrotic death of cardiomyocytes and provoke ischemia/reperfusion (I/R) injury. However, signaling pathways that regulate cardiomyocyte necrosis remain largely unknown. Our recent genome-wide RNAi screen has identified a potential necrosis
Sirinat Pengnet et al.
Frontiers in pharmacology, 13, 1049818-1049818 (2023-01-03)
Background: Excessive fructose consumption causes hepatic lipid accumulation via increased triglyceride (TG) synthesis, leading to the development and progression of non-alcoholic fatty liver disease (NALFD). Naringin, a flavanone glycoside found in citrus fruit, has antioxidant and hypolipidemic properties. Therefore, the
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico