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MAB1940

Sigma-Aldrich

Anti-Fibronectin Antibody, cellular, clone DH1

clone DH1, Chemicon®, from mouse

Sinónimos:

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

DH1, monoclonal

species reactivity

rat, guinea pig, human, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002026.2
NM_054034.2
NM_212474.1
NM_212475.1
NM_212476.1
NM_212478.1
NM_212482.1

UniProt accession no.

P02751

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
rabbit ... Fn1(100328589)
rat ... Fn1(25661)

Specificity

Specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin.

Immunogen

Epitope: cellular
Purified fibronectin from Human A8387 fibrosarcoma cells.

Application

Detect Fibronectin using this Anti-Fibronectin Antibody, cellular, clone DH1 validated for use in WB, IH.
Immunoblotting at 1:1,000

Indirect immunohistochemistry at 1:200-1:400

A 1-hour fixation with 3-3.5% paraformaldehyde in 0.1 M phosphate buffer pH 7.4 is suggested. Shows some reactivity on paraffin sections depending on fixation conditions and time (Latvala et al., 1996).

Final working dilutions must be determined by end user.

Physical form

Format: Purified
In PBS with 1% BSA and 0.1% sodium azide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Jassim M Al-Hassan et al.
Frontiers in pharmacology, 11, 899-899 (2020-07-07)
Preparations from Arabian Gulf catfish (Arius bilineatus, Val) epidermal gel secretion (PCEGS) effectively heal chronic wounds in diabetic patients. However, specific lipid components of PCEGS that are responsible for various aspects of wound healing are unknown. Here, we report for
Olaf Voets et al.
PloS one, 12(8), e0182974-e0182974 (2017-08-12)
Phenotypic assays using human primary cells are highly valuable tools for target discovery and validation in drug discovery. Expression knockdown (KD) of such targets in these assays allows the investigation of their role in models of disease processes. Therefore, efficient
Impaired elastogenesis in Hurler disease: dermatan sulfate accumulation linked to deficiency in elastin-binding protein and elastic fiber assembly.
Hinek, A; Wilson, SE
The American Journal of Pathology null
Iqra Mushtaq et al.
iScience, 27(6), 110084-110084 (2024-06-17)
The mutated SCN5A gene encoding defective Nav1.5 protein causes arrhythmic ailments and is associated with enhanced cardiac fibrosis. This study investigated whether SCN5A mutation directly affects cardiac fibroblasts and explored how defective SCN5A relates to cardiac fibrosis. SCN5A knockdown (SCN5AKD)
Grissel Faura Tellez et al.
American journal of physiology. Lung cellular and molecular physiology, 309(7), L725-L735 (2015-07-26)
Genetic studies have identified Protocadherin-1 (PCDH1) and Mothers against decapentaplegic homolog-3 (SMAD3) as susceptibility genes for asthma. PCDH1 is expressed in bronchial epithelial cells and has been found to interact with SMAD3 in yeast two-hybrid (Y2H) overexpression assays. Here, we
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