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Key Documents

MAB1576

Sigma-Aldrich

Anti-Muscarinic Acetylcholine Receptor m4 Antibody, clone 17F10.2

purified antibody, clone 17F10.2, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

17F10.2, monoclonal

species reactivity

mouse, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000741.2

UniProt accession no.

P08173

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Chrm4(12672)
rat ... Chrm4(25111)
rhesus monkey ... Chrm4(574195)

Specificity

m4 muscarinic acetylcholine receptor. No reactivity with the other subtypes.

Immunogen

m4 receptor i3 loop (human), fused to GST

Application

Immunohistochemistry (rat and monkey)

Western blot (rat, mouse, human): ECL 4-10 μg/mL

Immunoprecipitation (rat)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-Muscarinic Acetylcholine Receptor m4 Antibody, clone 17F10.2 is validated for use in IC, IP, WB for the detection of Muscarinic Acetylcholine Receptor m4.

Physical form

Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH=7.6 with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Keita Harada et al.
Acta histochemica et cytochemica, 56(4), 67-75 (2023-09-08)
Adrenal medullary chromaffin (AMC) and sympathetic ganglion cells are derived from the neural crest and show a similar developmental path. Thus, these two cell types have many common properties in membrane excitability and signaling. However, AMC cells function as endocrine
Taketsugu Hayashi et al.
Cell reports, 30(13), 4433-4444 (2020-04-03)
The ability to infer others' mental states is essential to social interactions. This ability, critically evaluated by testing whether one attributes false beliefs (FBs) to others, has been considered to be uniquely hominid and to accompany the activation of a
Lack of specificity of commercially available antisera: better specifications needed.
Pradidarcheep, W; Labruyere, WT; Dabhoiwala, NF; Lamers, WH
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Eleanor D Muise et al.
Data in brief, 10, 482-486 (2017-01-06)
The data presented in this article are related to the research article entitled "Distribution of muscarinic acetylcholine receptor subtypes in the murine small intestine" (E.D. Muise, N. Gandotra, J.J. Tackett, M.C. Bamdad, R.A. Cowles, 2016) [1]. We recently demonstrated that
Sinead M Clancy et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(24), 6388-6399 (2007-06-15)
Many inhibitory neurotransmitters in the brain activate Kir3 channels by stimulating pertussis toxin (PTX)-sensitive G-protein-coupled receptors. Here, we investigated the regulation of native muscarinic receptors and Kir3 channels expressed in NGF-differentiated PC12 cells, which are similar to sympathetic neurons. Quantitative
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